5LT8
Structure of the Epigenetic Oncogene MMSET and inhibition by N-Alkyl Sinefungin Derivatives
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-07-12 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.97 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 43.236, 76.836, 76.847 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.330 - 1.570 |
R-factor | 0.17623 |
Rwork | 0.174 |
R-free | 0.21551 |
Structure solution method | FOURIER SYNTHESIS |
RMSD bond length | 0.011 |
RMSD bond angle | 1.540 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | REFMAC |
Refinement software | REFMAC (5.8.0131) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 48.330 |
High resolution limit [Å] | 1.510 |
Number of reflections | 268230 |
<I/σ(I)> | 17.7 |
Completeness [%] | 99.7 |
Redundancy | 5.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1M HEPES, PH7.3, 0.1MKSCN, 25-30%MPEG2000 |