5LT5
Carboxysome shell protein CcmP from Synechococcus elongatus PCC 7942
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-03-02 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.97239 |
Spacegroup name | P 21 3 |
Unit cell lengths | 107.154, 107.154, 107.154 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.920 - 1.450 |
R-factor | 0.166 |
Rwork | 0.165 |
R-free | 0.18360 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4ht5 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.050 |
Data reduction software | XDS (November 11, 2013) |
Data scaling software | Aimless (0.1.29) |
Phasing software | PHASER (2.5.2) |
Refinement software | BUSTER-TNT (1.10.0) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 47.920 | 47.920 | 1.470 |
High resolution limit [Å] | 1.450 | 7.940 | 1.450 |
Rmerge | 0.058 | 0.028 | 0.893 |
Number of reflections | 72625 | ||
<I/σ(I)> | 21.7 | ||
Completeness [%] | 100.0 | 99.7 | 100 |
Redundancy | 9.9 | 9.5 | 9.6 |
CC(1/2) | 1.000 | 1.000 | 0.779 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 20 mM BIS-tris propane, 50 mM NaCl, 10 mM glycerol, 0.2 M Li sulphate, 0.1 mM Tris-HCl pH 7.5 and 5% w/v PEG 4000, 50 mM RuBP |