5LT3
nucleotide-free kinesin-1 motor domain T87A mutant, P1 crystal form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-11-27 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.979 |
Spacegroup name | P 1 |
Unit cell lengths | 56.672, 101.299, 101.527 |
Unit cell angles | 119.57, 91.91, 91.75 |
Refinement procedure
Resolution | 33.176 - 2.590 |
R-factor | 0.2061 |
Rwork | 0.203 |
R-free | 0.26300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4lnu |
RMSD bond length | 0.009 |
RMSD bond angle | 1.369 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.900 | |
High resolution limit [Å] | 2.590 | 2.590 |
Number of reflections | 59275 | |
<I/σ(I)> | 3.8 | |
Completeness [%] | 97.3 | |
Redundancy | 2.9 | |
CC(1/2) | 0.967 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 50 mM Mes pH 6.5 0.2M ammonium sulfate 30% mPEG 5000 |