5LT1
nucleotide-free kinesin-1 motor domain T92V mutant, P21 crystal form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-03-15 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 53.903, 69.921, 100.139 |
| Unit cell angles | 90.00, 100.81, 90.00 |
Refinement procedure
| Resolution | 43.353 - 1.949 |
| R-factor | 0.196 |
| Rwork | 0.195 |
| R-free | 0.23590 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1bg2 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.181 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.000 | |
| High resolution limit [Å] | 1.949 | |
| Rmeas | 0.180 | 1.180 |
| Number of reflections | 53434 | |
| <I/σ(I)> | 6.5 | |
| Completeness [%] | 99.7 | |
| Redundancy | 3.7 | |
| CC(1/2) | 0.992 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1 M Na-Acetate pH 4.5-5.5 23% PEG 4000 0.2 M Ammonium Sulfate 0.1 M SrCl2 |
