5LT0
nucleotide-free kinesin-1 motor domain, P212121 crystal form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-04-15 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.98 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 49.029, 68.058, 112.378 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 44.938 - 2.000 |
R-factor | 0.1771 |
Rwork | 0.174 |
R-free | 0.21540 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1bg2 |
RMSD bond length | 0.007 |
RMSD bond angle | 0.857 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX ((1.11rc1_2513: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.940 | |
High resolution limit [Å] | 2.000 | |
Rmeas | 0.170 | 1.380 |
Number of reflections | 26061 | |
<I/σ(I)> | 9.23 | |
Completeness [%] | 99.8 | |
Redundancy | 6.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 293 | 0.1 M Na-acetate pH 4.5-5.5 20-25% PEG 4000 0.2 M ammonium sulfate 0.1 mM SrCl2 |