5LSY
Structure of the Epigenetic Oncogene MMSET and inhibition by N-Alkyl Sinefungin Derivatives
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-06-17 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.976 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 58.983, 61.504, 77.308 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.890 - 1.620 |
| R-factor | 0.18304 |
| Rwork | 0.181 |
| R-free | 0.21469 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 3ooi |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.483 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.8.0131) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 46.893 |
| High resolution limit [Å] | 1.620 |
| Number of reflections | 182683 |
| <I/σ(I)> | 13.6 |
| Completeness [%] | 90.9 |
| Redundancy | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 293 | 0.1M Hepes, PH7.3, 0.1MKSCN, 25-30%MPEG2000 |
