5LSV
X-ray crystal structure of AA13 LPMO
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I911-3 |
| Synchrotron site | MAX II |
| Beamline | I911-3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-05-21 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 45.400, 58.840, 71.900 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.100 |
| R-factor | 0.10164 |
| Rwork | 0.101 |
| R-free | 0.12451 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4opb |
| RMSD bond length | 0.022 |
| RMSD bond angle | 2.038 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.130 |
| High resolution limit [Å] | 1.100 | 1.100 |
| Rmeas | 0.129 | 0.900 |
| Number of reflections | 78799 | |
| <I/σ(I)> | 13.37 | |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 7.44 | 6.76 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 298 | 20%(v/v)PEG3000 0.2M Zn-acetate 0.1 M Malate/MES/Tris pH5.0 |
