5LST
Crystal structure of the human RecQL4 helicase.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-07-07 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.9763 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 131.001, 131.001, 96.344 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 44.340 - 2.750 |
R-factor | 0.1853 |
Rwork | 0.183 |
R-free | 0.23140 |
Structure solution method | SAD |
RMSD bond length | 0.004 |
RMSD bond angle | 0.681 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | CRANK2 |
Refinement software | PHENIX (1.10_2155) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 44.340 | 2.900 |
High resolution limit [Å] | 2.750 | 2.750 |
Rmerge | 0.070 | 1.894 |
Number of reflections | 25149 | |
<I/σ(I)> | 21.7 | 1.4 |
Completeness [%] | 99.8 | 100 |
Redundancy | 14.3 | 14.3 |
CC(1/2) | 0.999 | 0.760 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 9 | 293.15 | Imidazole, Sodium formate |