5LSR
Carboxysome shell protein CcmP from Synechococcus elongatus PCC 7942
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-02-07 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.97625 |
Spacegroup name | I 21 3 |
Unit cell lengths | 178.740, 178.740, 178.740 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 44.680 - 1.650 |
R-factor | 0.1818 |
Rwork | 0.181 |
R-free | 0.19110 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4ht5 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.030 |
Data reduction software | XDS (November 3, 2014) |
Data scaling software | Aimless (0.1.29) |
Phasing software | PHASER (2.5.2) |
Refinement software | BUSTER-TNT (1.10.0) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 44.690 | 44.690 | 1.680 |
High resolution limit [Å] | 1.650 | 9.040 | 1.650 |
Rmerge | 0.093 | 0.071 | 1.404 |
Number of reflections | 113271 | ||
<I/σ(I)> | 18.9 | 48.3 | 2.3 |
Completeness [%] | 100.0 | 99.2 | 100 |
Redundancy | 20.1 | 19.7 | 17.6 |
CC(1/2) | 0.999 | 0.998 | 0.764 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 20 mM BIS-tris propane, 50 mM NaCl, 0.1 mM RuBP, 0.1 mM TrisHCl pH 7, 0.2 mM potassium thiocyanate, 6% poly-alpha-glutamic acid, |