5LSQ
Ethylene Forming Enzyme from Pseudomonas syringae pv. phaseolicola - I222 crystal form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04-1 |
Synchrotron site | Diamond |
Beamline | I04-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-11-16 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.91739 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 79.590, 97.750, 98.550 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.795 - 1.550 |
R-factor | 0.1356 |
Rwork | 0.135 |
R-free | 0.15660 |
Structure solution method | SAD |
RMSD bond length | 0.013 |
RMSD bond angle | 1.433 |
Data scaling software | Aimless (0.3.11) |
Phasing software | PHENIX |
Refinement software | PHENIX |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 39.795 | 2.030 | 1.590 |
High resolution limit [Å] | 1.550 | 1.970 | 1.550 |
Rmerge | 0.091 | 1.041 | 0.798 |
Number of reflections | 55915 | ||
<I/σ(I)> | 19.2 | 3 | |
Completeness [%] | 99.9 | 89.6 | 99.9 |
Redundancy | 13.2 | 12.3 | 13.8 |
CC(1/2) | 0.999 | 0.802 | 0.914 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 20% polyethylene glycol, 0.1 M MMT buffer, pH7.5, 0.05 Sodium/potatssium tartrate, protein concentration 20mg/mL, 0.003 MnCl2 |