5LSI

CRYSTAL STRUCTURE OF THE KINETOCHORE MIS12 COMPLEX HEAD2 SUBDOMAIN CONTAINING DSN1 AND NSL1 FRAGMENTS

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, DESY BEAMLINE P11
Synchrotron site	PETRA III, DESY
Beamline	P11
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-08-02
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.9793
Spacegroup name	P 32 2 1
Unit cell lengths	59.640, 59.640, 82.340
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	43.754 - 2.002
R-factor	0.2173
Rwork	0.216
R-free	0.24580
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5lsk
RMSD bond length	0.003
RMSD bond angle	0.516
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.10_2155)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	43.754		2.050
High resolution limit [Å]	2.000	8.950	2.000
Rmerge	0.242	0.046	1.355
Number of reflections	11845
<I/σ(I)>	10.63	41.65	2.12
Completeness [%]	99.9	99.4	99.4
Redundancy	19.1		18.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	5.6	293	2M ammonium sulfate, 0.2M potassium sodium phosphate, 0.1M sodium citrate pH 5.6