5LS9
Humanized Archaeal ferritin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-05-16 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.87292 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 185.797, 190.650, 176.537 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.690 - 2.930 |
| R-factor | 0.2784 |
| Rwork | 0.277 |
| R-free | 0.30190 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1s3q |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.998 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.000 |
| High resolution limit [Å] | 2.930 | 2.930 |
| Rmerge | 0.133 | 0.689 |
| Number of reflections | 59926 | |
| <I/σ(I)> | 7.8 | 1.8 |
| Completeness [%] | 96.0 | 96 |
| Redundancy | 4.2 | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277 | 22% Polyacrylic acid Tris pH 7.4 0.2 M MgCl2 |
