5LRI
PHOTOSYNTHETIC REACTION CENTER MUTANT WITH GLUL212 REPLACED WITH TRP (CHAIN L, EL212W)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX14.1 |
| Synchrotron site | SRS |
| Beamline | PX14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-11-11 |
| Detector | ADSC QUANTUM 4r |
| Wavelength(s) | 0.977 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 139.820, 139.820, 185.252 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 17.910 - 2.400 |
| R-factor | 0.19044 |
| Rwork | 0.189 |
| R-free | 0.21749 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Wild Type RHODOBACTER SPHAEROIDESCOORDINATES (UNPUBLISHED DATA) |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.763 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0131) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 18.000 | 2.440 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.057 | 0.500 |
| Number of reflections | 77800 | |
| <I/σ(I)> | 16.4 | 1.63 |
| Completeness [%] | 95.0 | 95.6 |
| Redundancy | 4.2 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 291 | Well containing 9 mg mL-1 RC, 0.09 % v/v LDAO, 3.5 % w/v 1,2,3-heptanetriol, and 0.75 M potassium phosphate (pH 7.5) equilibrated against 1.5 M potassium phosphate |
