5LRB
Plastidial phosphorylase from Barley in complex with acarbose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-07-19 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.95373 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 230.330, 63.680, 149.330 |
| Unit cell angles | 90.00, 115.07, 90.00 |
Refinement procedure
| Resolution | 48.920 - 2.900 |
| R-factor | 0.21358 |
| Rwork | 0.213 |
| R-free | 0.24602 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5lr8 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.107 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.100 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.137 | 1.153 |
| Number of reflections | 43219 | |
| <I/σ(I)> | 7.6 | 0.98 |
| Completeness [%] | 98.0 | |
| Redundancy | 3.2 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.8 | 288 | 30% PEG4000, 0.1M Nacitrate |
