5LRA
Plastidial phosphorylase PhoI from barley in complex with maltotetraose
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-2 |
Synchrotron site | ESRF |
Beamline | ID23-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-11-14 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.87260 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 227.690, 63.150, 148.190 |
Unit cell angles | 90.00, 114.72, 90.00 |
Refinement procedure
Resolution | 49.390 - 3.000 |
R-factor | 0.19626 |
Rwork | 0.195 |
R-free | 0.24286 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5lrb |
RMSD bond length | 0.006 |
RMSD bond angle | 1.158 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.200 |
High resolution limit [Å] | 3.000 | 3.000 |
Rmerge | 0.109 | 1.128 |
Number of reflections | 38803 | |
<I/σ(I)> | 10.1 | 1.13 |
Completeness [%] | 99.7 | |
Redundancy | 3.8 | 3.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 288 | 30% PEG4000, 0.1M Na Citrate |