5LR3

RNA duplex has central consecutive GA pairs flanked by G-U basepairs

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I02
Synchrotron site	Diamond
Beamline	I02
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-02-05
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9796
Spacegroup name	P 43 21 2
Unit cell lengths	33.300, 33.300, 120.910
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.168 - 1.650
R-factor	0.2484
Rwork	0.247
R-free	0.26560
Structure solution method	SAD
RMSD bond length	0.001
RMSD bond angle	2.499
Data reduction software	xia2
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX ((dev_2481: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.170	1.690
High resolution limit [Å]	1.650	1.650
Rmerge	0.063	1.900
Number of reflections	8761
<I/σ(I)>	16.8	1
Completeness [%]	98.5	95.2
Redundancy	9.9	10.2
CC(1/2)	1.000	0.600

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	293	0.015 M Magnesium Acetate, 0.05 M Sodium Cacodylate pH 6.0, 1.7 M Ammonium Sulfate