5LQO
RNA duplex has central consecutive GA pairs flanked by G-C basepairs
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I02 |
Synchrotron site | Diamond |
Beamline | I02 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-02-15 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9196 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 33.469, 33.469, 113.766 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 32.108 - 1.870 |
R-factor | 0.2461 |
Rwork | 0.246 |
R-free | 0.25560 |
Structure solution method | SAD |
RMSD bond length | 0.005 |
RMSD bond angle | 1.458 |
Data reduction software | xia2 |
Data scaling software | Aimless |
Phasing software | PHENIX |
Refinement software | PHENIX ((dev_2481: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 33.470 | 1.920 |
High resolution limit [Å] | 1.870 | 1.870 |
Rmerge | 0.072 | 2.080 |
Number of reflections | 5917 | |
<I/σ(I)> | 10.7 | 1.3 |
Completeness [%] | 100.0 | 100 |
Redundancy | 12.2 | 13 |
CC(1/2) | 1.000 | 0.446 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 0.025 M Magnesium Sulfate, 0.05 M Tris Hydrochloride pH 8.5, 1.8 M Ammonium Sulfate |