5LQ6
Salmonella effector SpvD - R161 variant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-11-21 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97625 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 43.700, 51.810, 47.270 |
| Unit cell angles | 90.00, 107.41, 90.00 |
Refinement procedure
| Resolution | 45.100 - 1.480 |
| R-factor | 0.17114 |
| Rwork | 0.170 |
| R-free | 0.20132 |
| Structure solution method | SIRAS |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.578 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.8.0107) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 51.810 | 1.560 |
| High resolution limit [Å] | 1.480 | 1.480 |
| Rmerge | 0.076 | |
| Number of reflections | 33404 | |
| <I/σ(I)> | 12.1 | 2.1 |
| Completeness [%] | 99.1 | 94.1 |
| Redundancy | 5.9 | 4.6 |
| CC(1/2) | 0.532 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | 100 mM Tris, 200 mM NaCl, 22 % PEG3350 |
