5LQ0
Crystal structure of Tyr24 phosphorylated Annexin A2 at 2.9 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-12-06 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 56.450, 63.470, 268.910 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.767 - 2.900 |
| R-factor | 0.2282 |
| Rwork | 0.227 |
| R-free | 0.25980 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2hyw |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.627 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.770 | 3.000 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Number of reflections | 22187 | |
| <I/σ(I)> | 18.67 | 3.22 |
| Completeness [%] | 99.1 | 99.86 |
| Redundancy | 9.14 | 6.33 |
| CC(1/2) | 0.999 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 296 | 0.1 M MES pH 6, 0.1 M magnesium chloride, 4% PEG6000 |
