5LPY
Crystal structure of the BRI1 kinase domain (865-1160) in complex with ATP from Arabidopsis thaliana
Replaces: 4OABExperimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-08-19 |
Detector | DECTRIS PILATUS 2M |
Wavelength(s) | 1.000040 |
Spacegroup name | P 62 |
Unit cell lengths | 115.823, 115.823, 49.898 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 44.670 - 2.300 |
R-factor | 0.2031 |
Rwork | 0.201 |
R-free | 0.24800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3uim |
RMSD bond length | 0.005 |
RMSD bond angle | 0.714 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 44.670 | 2.440 |
High resolution limit [Å] | 2.300 | 2.300 |
Number of reflections | 17194 | |
<I/σ(I)> | 36.77 | 2.49 |
Completeness [%] | 99.7 | 98.8 |
Redundancy | 14.2 | 14.3 |
CC(1/2) | 1.000 | 0.767 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 28% PEG 20K/MME 550 morpheus mix, 0.12 M eth glycol mix, 0.1 M Hepes pH 7.5 soaked with 30 mM ATP in xtal condition (around 3 min) |