5LPY

Crystal structure of the BRI1 kinase domain (865-1160) in complex with ATP from Arabidopsis thaliana

Replaces:  4OAB

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-08-19
Detector	DECTRIS PILATUS 2M
Wavelength(s)	1.000040
Spacegroup name	P 62
Unit cell lengths	115.823, 115.823, 49.898
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.670 - 2.300
R-factor	0.2031
Rwork	0.201
R-free	0.24800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3uim
RMSD bond length	0.005
RMSD bond angle	0.714
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.670	2.440
High resolution limit [Å]	2.300	2.300
Number of reflections	17194
<I/σ(I)>	36.77	2.49
Completeness [%]	99.7	98.8
Redundancy	14.2	14.3
CC(1/2)	1.000	0.767

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	298	28% PEG 20K/MME 550 morpheus mix, 0.12 M eth glycol mix, 0.1 M Hepes pH 7.5 soaked with 30 mM ATP in xtal condition (around 3 min)