5LPX
Crystal structure of PKC phosphorylation-mimicking mutant (S26E) Annexin A2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-12-06 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 54.950, 58.760, 182.090 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.045 - 1.900 |
| R-factor | 0.1473 |
| Rwork | 0.145 |
| R-free | 0.18210 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2hyw |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.469 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.050 | 1.960 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 47503 | |
| <I/σ(I)> | 19.05 | 2.3 |
| Completeness [%] | 100.0 | 99.98 |
| Redundancy | 13.18 | |
| CC(1/2) | 0.999 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 296 | 0.1 M Bis-Tris pH 6, 0.2 M ammonium acetate, 5% PEG 3350 |
