5LPV
Crystal structure of the BRI1 kinase domain (865-1160) in complex with AMPPNP and Mn from Arabidopsis thaliana
Replaces: 4OA9Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-08-19 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.000040 |
Spacegroup name | P 62 |
Unit cell lengths | 115.630, 115.630, 49.640 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 44.475 - 2.700 |
R-factor | 0.1929 |
Rwork | 0.191 |
R-free | 0.23280 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3uim |
RMSD bond length | 0.002 |
RMSD bond angle | 0.597 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 44.480 | 2.860 |
High resolution limit [Å] | 2.700 | 2.700 |
Number of reflections | 10619 | |
<I/σ(I)> | 1 | 2.35 |
Completeness [%] | 97.9 | 97.9 |
Redundancy | 12.09 | 11.98 |
CC(1/2) | 0.851 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 28% PEG 20K/MME 550 morpheus mix, 0.12 M eth glycol mix, 0.1 M Hepes pH 6.5 |