5LPT
tRNA guanine Transglycosylase (TGT) in co-crystallized complex (space group P21) with 6-amino-2-(methylamino)-4-(2-((2R,3R,4S,5R,6S)-3,4,5,6-tetramethoxytetrahydro-2H-pyran-2-yl)ethyl)-1H-imidazo[4,5-g]quinazolin-8(7H)-one
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-10-24 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.918409 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 68.745, 78.866, 84.891 |
Unit cell angles | 90.00, 108.72, 90.00 |
Refinement procedure
Resolution | 40.510 - 2.360 |
R-factor | 0.2026 |
Rwork | 0.201 |
R-free | 0.23340 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1p0d |
RMSD bond length | 0.006 |
RMSD bond angle | 0.702 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10.1_2155: 1492)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.290 | 2.500 |
High resolution limit [Å] | 2.360 | 2.360 |
Rmerge | 0.495 | |
Number of reflections | 35384 | |
<I/σ(I)> | 8.1 | 2.6 |
Completeness [%] | 99.0 | 96.6 |
Redundancy | 3.4 | 3.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 288 | 13% PEG 8000, 100mM MES, 1mM DTT, 10% DMSO |