5LPN

Structure of human Rab10 in complex with the bMERB domain of Mical-1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-09-20
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.99997
Spacegroup name	P 21 21 21
Unit cell lengths	58.390, 59.000, 198.180
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.004 - 2.800
R-factor	0.2462
Rwork	0.244
R-free	0.28660
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4lhw and 5szg
RMSD bond length	0.008
RMSD bond angle	1.118
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.10_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.004	2.900
High resolution limit [Å]	2.800	2.800
Rmerge	0.118	0.723
Number of reflections	17508
<I/σ(I)>	14.12	3.25
Completeness [%]	99.6	99.9
Redundancy	12.7	12.6
CC(1/2)		0.803

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	6%-10% PEG8000 0.1M Imidazole