5LPF

Kallikrein-related peptidase 10

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	MPG/DESY, HAMBURG BEAMLINE BW6
Synchrotron site	MPG/DESY, HAMBURG
Beamline	BW6
Temperature [K]	100
Detector technology	CCD
Collection date	2004-07-26
Detector	MAR CCD 165 mm
Wavelength(s)	1.05000
Spacegroup name	P 1 21 1
Unit cell lengths	53.602, 66.841, 57.900
Unit cell angles	90.00, 104.29, 90.00

Refinement procedure

Resolution	36.694 - 2.700
R-factor	0.2268
Rwork	0.223
R-free	0.28490
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2bdg
RMSD bond length	0.011
RMSD bond angle	1.128
Data reduction software	MOSFLM
Data scaling software	Aimless (0.5.27)
Phasing software	PHASER (2.6.1)
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	51.940	2.830
High resolution limit [Å]	2.700	2.700
Rmerge	0.065	0.180
Number of reflections	10481
<I/σ(I)>	14.4	2.989
Completeness [%]	95.0	93.3
Redundancy	3.3	2.4
CC(1/2)	0.989	0.947

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.6	291	0.1 M ammonium sulphate, 0.1 M MES