5LPF
Kallikrein-related peptidase 10
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | MPG/DESY, HAMBURG BEAMLINE BW6 |
Synchrotron site | MPG/DESY, HAMBURG |
Beamline | BW6 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-07-26 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 1.05000 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 53.602, 66.841, 57.900 |
Unit cell angles | 90.00, 104.29, 90.00 |
Refinement procedure
Resolution | 36.694 - 2.700 |
R-factor | 0.2268 |
Rwork | 0.223 |
R-free | 0.28490 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2bdg |
RMSD bond length | 0.011 |
RMSD bond angle | 1.128 |
Data reduction software | MOSFLM |
Data scaling software | Aimless (0.5.27) |
Phasing software | PHASER (2.6.1) |
Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 51.940 | 2.830 |
High resolution limit [Å] | 2.700 | 2.700 |
Rmerge | 0.065 | 0.180 |
Number of reflections | 10481 | |
<I/σ(I)> | 14.4 | 2.989 |
Completeness [%] | 95.0 | 93.3 |
Redundancy | 3.3 | 2.4 |
CC(1/2) | 0.989 | 0.947 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 291 | 0.1 M ammonium sulphate, 0.1 M MES |