5LPE

Kallikrein-related peptidase 10 complex with Zn2+

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	MPG/DESY, HAMBURG BEAMLINE BW6
Synchrotron site	MPG/DESY, HAMBURG
Beamline	BW6
Temperature [K]	100
Detector technology	CCD
Collection date	2004-07-26
Detector	MAR CCD 165 mm
Wavelength(s)	1.05000
Spacegroup name	P 1 21 1
Unit cell lengths	52.914, 67.037, 57.768
Unit cell angles	90.00, 104.37, 90.00

Refinement procedure

Resolution	43.565 - 2.650
R-factor	0.2229
Rwork	0.220
R-free	0.27770
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2bdg
RMSD bond length	0.011
RMSD bond angle	1.433
Data reduction software	MOSFLM
Data scaling software	Aimless (0.5.27)
Phasing software	PHASER (2.6.1)
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	55.960	2.780
High resolution limit [Å]	2.650	2.650
Rmerge	0.104	0.594
Number of reflections	11339
<I/σ(I)>	8.1	0.975
Completeness [%]	98.7	99.9
Redundancy	3.1	3.1
CC(1/2)	0.991	0.715

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.6	291	100 mM ammonium sulphate, 100 mM MES