5LPE
Kallikrein-related peptidase 10 complex with Zn2+
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | MPG/DESY, HAMBURG BEAMLINE BW6 |
Synchrotron site | MPG/DESY, HAMBURG |
Beamline | BW6 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-07-26 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 1.05000 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 52.914, 67.037, 57.768 |
Unit cell angles | 90.00, 104.37, 90.00 |
Refinement procedure
Resolution | 43.565 - 2.650 |
R-factor | 0.2229 |
Rwork | 0.220 |
R-free | 0.27770 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2bdg |
RMSD bond length | 0.011 |
RMSD bond angle | 1.433 |
Data reduction software | MOSFLM |
Data scaling software | Aimless (0.5.27) |
Phasing software | PHASER (2.6.1) |
Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 55.960 | 2.780 |
High resolution limit [Å] | 2.650 | 2.650 |
Rmerge | 0.104 | 0.594 |
Number of reflections | 11339 | |
<I/σ(I)> | 8.1 | 0.975 |
Completeness [%] | 98.7 | 99.9 |
Redundancy | 3.1 | 3.1 |
CC(1/2) | 0.991 | 0.715 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 291 | 100 mM ammonium sulphate, 100 mM MES |