5LPC
Crystal structure of Vanadium-dependent Haloperoxidase from A. marina
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-09-23 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | F 41 3 2 |
| Unit cell lengths | 306.160, 306.160, 306.160 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.000 - 3.100 |
| R-factor | 0.20949 |
| Rwork | 0.207 |
| R-free | 0.24941 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1up8 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.909 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.200 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Rmerge | 0.092 | 0.572 |
| Number of reflections | 21760 | |
| <I/σ(I)> | 11.2 | 2.2 |
| Completeness [%] | 95.2 | 96.9 |
| Redundancy | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 0.1 M TRIS, 1.25 M Ammoniumdihydrogenphosphate, 0.5 mM potassium vanadate |
