5LPB
Crystal structure of the BRI1 kinase domain (865-1160) in complex with ADP from Arabidopsis thaliana
Replaces: 4OACExperimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-07-06 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.97625 |
| Spacegroup name | P 62 |
| Unit cell lengths | 116.141, 116.141, 49.743 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 44.580 - 1.980 |
| R-factor | 0.1838 |
| Rwork | 0.183 |
| R-free | 0.20320 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3uim |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.792 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.580 | 2.100 |
| High resolution limit [Å] | 1.980 | 1.980 |
| Number of reflections | 26940 | |
| <I/σ(I)> | 24.28 | 1.71 |
| Completeness [%] | 99.9 | 99.6 |
| Redundancy | 10.2 | 10.1 |
| CC(1/2) | 1.000 | 0.836 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 28% PEG 20K/MME 550 morpheus mix 0.12 M eth glycol mix 0.1 M Hepes pH 7 1mM ADP |
