5LP0
CRYSTAL STRUCTURE OF THE ZEBRA FISH ENTH DOMAIN FROM EPSIN1 IN 1.41 ANGSTROM RESOLUTION
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2011-04-20 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97881 |
Spacegroup name | P 1 |
Unit cell lengths | 42.364, 51.724, 64.639 |
Unit cell angles | 89.33, 76.37, 79.74 |
Refinement procedure
Resolution | 40.490 - 1.410 |
R-factor | 0.157 |
Rwork | 0.154 |
R-free | 0.20200 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1h0a |
RMSD bond length | 0.009 |
RMSD bond angle | 1.134 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.8_1069) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 62.788 | 1.460 |
High resolution limit [Å] | 1.410 | 1.410 |
Rmerge | 0.075 | 0.320 |
Number of reflections | 95136 | |
<I/σ(I)> | 8.6 | |
Completeness [%] | 94.0 | 91.4 |
Redundancy | 3.4 | 3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 292 | 19% (W/V) PEG 3350, 0.1M POTASSIUM PHOSPHATE MONOBASIC MONOHYDRATE, PH 7.0, VAPOR DIFFUSION, SITTING DROP, TEMPERATURE 292K |