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5LP0

CRYSTAL STRUCTURE OF THE ZEBRA FISH ENTH DOMAIN FROM EPSIN1 IN 1.41 ANGSTROM RESOLUTION

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsESRF BEAMLINE ID29
Synchrotron siteESRF
BeamlineID29
Temperature [K]100
Detector technologyPIXEL
Collection date2011-04-20
DetectorDECTRIS PILATUS 6M
Wavelength(s)0.97881
Spacegroup nameP 1
Unit cell lengths42.364, 51.724, 64.639
Unit cell angles89.33, 76.37, 79.74
Refinement procedure
Resolution40.490 - 1.410
R-factor0.157
Rwork0.154
R-free0.20200
Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)1h0a
RMSD bond length0.009
RMSD bond angle1.134
Data reduction softwareHKL-2000
Data scaling softwareHKL-2000
Phasing softwarePHASER
Refinement softwarePHENIX (1.8_1069)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]62.7881.460
High resolution limit [Å]1.4101.410
Rmerge0.0750.320
Number of reflections95136
<I/σ(I)>8.6
Completeness [%]94.091.4
Redundancy3.43
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, SITTING DROP729219% (W/V) PEG 3350, 0.1M POTASSIUM PHOSPHATE MONOBASIC MONOHYDRATE, PH 7.0, VAPOR DIFFUSION, SITTING DROP, TEMPERATURE 292K

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