5LOI
Crystal structure of Myceliophthora thermophila Rad26 (residues 373-841)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-07-14 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.979 |
Spacegroup name | I 4 2 2 |
Unit cell lengths | 210.783, 210.783, 104.766 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 105.391 - 3.153 |
R-factor | 0.2101 |
Rwork | 0.207 |
R-free | 0.23510 |
Structure solution method | SAD |
RMSD bond length | 0.009 |
RMSD bond angle | 1.010 |
Data reduction software | XDS |
Phasing software | SHELXCD |
Refinement software | PHENIX ((dev_2614: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 105.400 | 3.270 |
High resolution limit [Å] | 3.150 | 3.150 |
Rmerge | 0.123 | 4.781 |
Rpim | 0.025 | 0.937 |
Number of reflections | 20640 | 1932 |
<I/σ(I)> | 23 | 0.8 |
Completeness [%] | 99.4 | 95.07 |
Redundancy | 26.2 | 26.8 |
CC(1/2) | 0.999 | 0.612 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 291 | 0.1M BIS-TRIS pH 6.5 10% (w/v) PEG 10K 0.2M Potassium sodium tartrate |