5LOG
Crystal Structure of SafC from Myxococcus xanthus bound to SAM
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-08-24 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 1.000030 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 60.840, 51.017, 72.677 |
| Unit cell angles | 90.00, 98.58, 90.00 |
Refinement procedure
| Resolution | 71.860 - 2.010 |
| R-factor | 0.178 |
| Rwork | 0.176 |
| R-free | 0.21200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2hnk |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.020 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.5.23) |
| Phasing software | MOLREP |
| Refinement software | BUSTER-TNT (2.10.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 71.860 | 2.110 |
| High resolution limit [Å] | 2.005 | 2.005 |
| Rmerge | 0.303 | 1.159 |
| Rmeas | 0.357 | |
| Rpim | 0.137 | |
| Total number of observations | 193880 | |
| Number of reflections | 28956 | |
| <I/σ(I)> | 7 | 2.1 |
| Completeness [%] | 97.0 | 90.4 |
| Redundancy | 6.7 | 6.8 |
| CC(1/2) | 0.980 | 0.373 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 298 | PEG MME 5000, Sodium Acetate pH 5.5 |
