5LOC
Crystal structure of the engineered D-Amino Acid Dehydrogenase (DAADH)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-07-12 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.9282 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 120.530, 120.530, 97.630 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 71.302 - 2.040 |
R-factor | 0.209 |
Rwork | 0.207 |
R-free | 0.25450 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.319 |
Data reduction software | XDS |
Data scaling software | xia2 |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 71.302 |
High resolution limit [Å] | 2.040 |
Number of reflections | 529247 |
<I/σ(I)> | 17.5 |
Completeness [%] | 99.6 |
Redundancy | 10.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 277 | 0.12 M monosaccharides, 0.1 M Buffer System 3 pH 8.5, 30%, P500MME_P20K |