5LO8
The C2B domain of Rabphilin 3A in complex with PI(4,5)P2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-07-21 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 4 |
| Unit cell lengths | 92.520, 92.520, 40.020 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 65.422 - 2.500 |
| R-factor | 0.271 |
| Rwork | 0.252 |
| R-free | 0.26460 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2cm5 |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.592 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA (ccp4i) |
| Phasing software | PHASER (ccp4i) |
| Refinement software | PHENIX ((dev_2666: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 65.422 | 2.640 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.162 | 0.766 |
| Number of reflections | 11782 | |
| <I/σ(I)> | 5.5 | 1.3 |
| Completeness [%] | 98.3 | 96.1 |
| Redundancy | 3 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 24% PEG3350 0.1M ammonium sulfate 50mM Tris pH 7.5 |
