5LNX
Crystal structure of MmgC, an acyl-CoA dehydrogenase from bacillus subtilis.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-04-14 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9763 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 78.168, 279.266, 78.191 |
| Unit cell angles | 90.00, 101.40, 90.00 |
Refinement procedure
| Resolution | 48.760 - 2.600 |
| R-factor | 0.2489 |
| Rwork | 0.247 |
| R-free | 0.28462 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.643 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 48.760 |
| High resolution limit [Å] | 2.600 |
| Number of reflections | 100379 |
| <I/σ(I)> | 8.5 |
| Completeness [%] | 100.0 |
| Redundancy | 6.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 291 | 0.1 M MMT pH 8, 25% PEG 1500 and 20 % glycerol |
