5LNT

Crystal structure of Arabidopsis thaliana Pdx1K166R-preI320 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	CCD
Collection date	2008-09-04
Detector	ADSC QUANTUM 210
Wavelength(s)	0.933
Spacegroup name	H 3
Unit cell lengths	177.900, 177.900, 115.020
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	24.736 - 2.320
R-factor	0.1501
Rwork	0.148
R-free	0.19590
Structure solution method	FOURIER SYNTHESIS
RMSD bond length	0.007
RMSD bond angle	0.838
Data scaling software	SCALEPACK
Refinement software	PHENIX (1.10_2155)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	25.000	25.000	2.350
High resolution limit [Å]	2.320	6.740	2.320
Rmerge	0.095	0.055	0.336
Total number of observations	331737
Number of reflections	58683
<I/σ(I)>	25.1
Completeness [%]	99.9	99.6	100
Redundancy	5.7	5.7	5.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	298	0.1 M sodium cacodylate pH6.5, 0.2 M ammonium sulfate, 15%(w/v) PEG8000