5LNR

Crystal structure of Arabidopsis thaliana Pdx1-PLP complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-1
Synchrotron site	ESRF
Beamline	ID14-1
Temperature [K]	100
Detector technology	CCD
Collection date	2011-12-04
Detector	ADSC QUANTUM 210
Wavelength(s)	0.93340
Spacegroup name	H 3
Unit cell lengths	178.560, 178.560, 116.881
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	40.264 - 1.610
R-factor	0.1252
Rwork	0.123
R-free	0.16450
Structure solution method	FOURIER SYNTHESIS
RMSD bond length	0.005
RMSD bond angle	0.709
Data scaling software	Aimless (0.5.17)
Refinement software	PHENIX (1.10_2155)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	40.260	40.260	1.640
High resolution limit [Å]	1.610	8.820	1.610
Rmerge	0.049	0.032	0.475
Rmeas	0.060	0.040	0.589
Rpim	0.035	0.023	0.345
Total number of observations	510741	2919	25085
Number of reflections	179995
<I/σ(I)>	9	23.1	1.5
Completeness [%]	100.0	96.9	100
Redundancy	2.8	2.8	2.8
CC(1/2)	0.998	0.996	0.799

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.25	298	0.1 M Tris pH8.25, 0.2 M sodium acetate pH5.5, 12.3%(w/v) PEG4000