5LND
Crystal structure of self-complemented MyfA, the major subunit of Myf fimbriae from Yersinia enterocolitica
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-12-07 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97625 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 41.970, 41.979, 151.185 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.449 - 1.460 |
| R-factor | 0.1853 |
| Rwork | 0.181 |
| R-free | 0.19360 |
| Structure solution method | MAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.996 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 41.979 | 41.979 | 1.510 |
| High resolution limit [Å] | 1.460 | 5.650 | 1.460 |
| Rmerge | 0.049 | 0.474 | |
| Rmeas | 0.074 | 0.057 | 0.561 |
| Rpim | 0.038 | 0.029 | 0.292 |
| Total number of observations | 149827 | 2841 | 15115 |
| Number of reflections | 45504 | ||
| <I/σ(I)> | 10.1 | 18 | 2.2 |
| Completeness [%] | 96.7 | 92.1 | 98.7 |
| Redundancy | 3.3 | 3.3 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 294 | 35% dioxane v/v |
