5LNC
Structure of SPX domain of the yeast inorganic polyphophate polymerase Vtc4 crystallized by carrier-driven crystallization in fusion with the macro domain of human histone macroH2A1.1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-05-05 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97963 |
| Spacegroup name | I 21 21 21 |
| Unit cell lengths | 114.020, 130.050, 158.330 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.900 - 3.290 |
| R-factor | 0.28 |
| Rwork | 0.279 |
| R-free | 0.30200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1yd9 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.130 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.900 | 3.380 |
| High resolution limit [Å] | 3.290 | 3.290 |
| Number of reflections | 17957 | |
| <I/σ(I)> | 15.37 | 0.6 |
| Completeness [%] | 98.4 | 82.3 |
| Redundancy | 7.1 | 5.3 |
| CC(1/2) | 1.000 | 0.253 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 8.75% PEG 6000, 2.75M NaCl |
