5LN8

Crystal structure of self-complemented MyfA, the major subunit of Myf fimbriae from Yersinia enterocolitica, in complex with galactose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-2
Synchrotron site	ESRF
Beamline	ID23-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-11-07
Detector	DECTRIS PILATUS3 2M
Wavelength(s)	0.8726
Spacegroup name	P 21 21 21
Unit cell lengths	41.840, 41.930, 151.140
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.042 - 1.650
R-factor	0.1741
Rwork	0.173
R-free	0.19860
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5lnd
RMSD bond length	0.006
RMSD bond angle	0.979
Data reduction software	SCALA
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX (1.8.2_1309)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	28.070	1.740
High resolution limit [Å]	1.650	1.650
Rmerge	0.129	0.750
Number of reflections	32729
<I/σ(I)>	8	2
Completeness [%]	99.3	98.6
Redundancy	5.5	4.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		294	35% dioxane v/v