5LMZ

Fluorinase from Streptomyces sp. MA37

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	Cu FINE FOCUS
Temperature [K]	100
Detector technology	CCD
Collection date	2013-06-26
Detector	RIGAKU SATURN 944
Wavelength(s)	1.54
Spacegroup name	P 21 3
Unit cell lengths	125.920, 125.920, 125.920
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	18.170 - 2.550
R-factor	0.1686
Rwork	0.166
R-free	0.21580
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2c2w
RMSD bond length	0.007
RMSD bond angle	0.978
Data reduction software	iMOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX (1.8.1_1168)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	18.170	2.690
High resolution limit [Å]	2.550	2.550
Rmerge	0.200	0.800
Number of reflections	21982
<I/σ(I)>	11.4	3
Completeness [%]	99.7	100
Redundancy	11.9	11.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.5 M NaCl, 7 % w/v PEG6000