5LMV
Structure of bacterial 30S-IF1-IF2-IF3-mRNA-tRNA translation pre-initiation complex(state-III)
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK I |
EM Data Collection
| Microscope | FEI TITAN KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Cs (mm) | 2.7 |
| Detector | FEI FALCON II (4k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 4.9 |
| Number of Particle | 803433 |
| Software | RELION,REFMAC,EMAN,EPU,CTFFIND,UCSF Chimera,Coot |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
