5LMO
Structure of bacterial 30S-IF1-IF3-mRNA translation pre-initiation complex (state-1B)
EM specimen
Vitrification | Yes |
Instrument | FEI VITROBOT MARK I |
EM Data Collection
Microscope | FEI POLARA 300 |
Electron Gun | FIELD EMISSION GUN |
Accelerating Voltage (kV) | 300 |
Cs (mm) | 2 |
Detector | OTHER |
3D Reconstruction
Method | SINGLE PARTICLE |
Resolution (Å) | 4.3 |
Number of Particle | 666610 |
Software | RELION,REFMAC,EMAN,EPU,CTFFIND,UCSF Chimera,Coot |
CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |