5LMJ
Llama nanobody PorM_19
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-06-05 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.07224 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 113.258, 113.258, 153.486 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.630 - 2.100 |
| R-factor | 0.189 |
| Rwork | 0.188 |
| R-free | 0.21000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4hem |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.110 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | BUSTER (2.10.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.100 | 2.210 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.075 | 1.353 |
| Number of reflections | 58799 | |
| <I/σ(I)> | 15 | 1.7 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 8.7 | 8.2 |
| CC(1/2) | 0.998 | 0.652 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1M Hepes pH4.5-5.5 0.4-1.2M NaH2PO4 0.4-1.2M KH2PO4 |
