5LMH
High dose Thaumatin - 160-200 ms.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2) |
Synchrotron site | PETRA III, EMBL c/o DESY |
Beamline | P14 (MX2) |
Temperature [K] | 296 |
Detector technology | PIXEL |
Collection date | 2015-10-24 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.96863 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 58.480, 58.480, 151.730 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.950 - 1.960 |
R-factor | 0.1808 |
Rwork | 0.179 |
R-free | 0.22300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1lr2 |
RMSD bond length | 0.015 |
RMSD bond angle | 1.691 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0151) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.950 | 2.030 |
High resolution limit [Å] | 1.960 | 1.960 |
Rmerge | 0.143 | 0.663 |
Number of reflections | 18364 | |
<I/σ(I)> | 5.8 | 1.9 |
Completeness [%] | 93.1 | 93.4 |
Redundancy | 3.2 | 3.3 |
CC(1/2) | 0.986 | 0.650 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 296 | .3 M sodium tartrate and 50 mM Tris, pH 6.8 |