5LMH
High dose Thaumatin - 160-200 ms.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2) |
| Synchrotron site | PETRA III, EMBL c/o DESY |
| Beamline | P14 (MX2) |
| Temperature [K] | 296 |
| Detector technology | PIXEL |
| Collection date | 2015-10-24 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.96863 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 58.480, 58.480, 151.730 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.950 - 1.960 |
| R-factor | 0.1808 |
| Rwork | 0.179 |
| R-free | 0.22300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1lr2 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.691 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0151) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.950 | 2.030 |
| High resolution limit [Å] | 1.960 | 1.960 |
| Rmerge | 0.143 | 0.663 |
| Number of reflections | 18364 | |
| <I/σ(I)> | 5.8 | 1.9 |
| Completeness [%] | 93.1 | 93.4 |
| Redundancy | 3.2 | 3.3 |
| CC(1/2) | 0.986 | 0.650 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 296 | .3 M sodium tartrate and 50 mM Tris, pH 6.8 |
