5LM8

Crystal structure of a laccase-like multicopper oxidase McoG from from Aspergillus niger

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-07-17
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.960
Spacegroup name	C 1 2 1
Unit cell lengths	200.709, 61.346, 53.836
Unit cell angles	90.00, 95.29, 90.00

Refinement procedure

Resolution	29.440 - 1.700
R-factor	0.1751
Rwork	0.173
R-free	0.22152
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1gw0
RMSD bond length	0.021
RMSD bond angle	2.083
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP (10.2.23)
Refinement software	REFMAC (5.8.0073)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	29.571	29.571	1.800
High resolution limit [Å]	1.690	5.020	1.690
Rmerge	0.069	0.046	0.307
Number of reflections	65942
<I/σ(I)>	9.11	21.17	2.05
Completeness [%]	90.5	90.5	68.5
Redundancy	2.6
CC(1/2)	0.995	0.992	0.947

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	5.5	296	20% PEG 3350, 0.2 M NaCl, 0.1 M sodium citrate