5LLW
Bacteriophytochrome activated diguanylyl cyclase from Idiomarina species A28L
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-11-19 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.979168 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 50.870, 77.800, 439.980 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 63.518 - 3.000 |
| R-factor | 0.2072 |
| Rwork | 0.205 |
| R-free | 0.25870 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.121 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SOLVE |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 68.700 | 2.900 | |
| High resolution limit [Å] | 2.800 | 20.000 | 2.800 |
| Rmerge | 0.132 | 0.054 | |
| Number of reflections | 67335 | ||
| <I/σ(I)> | 15.85 | 47.64 | |
| Completeness [%] | 81.1 | 96.1 | |
| Redundancy | 14.94 | ||
| CC(1/2) | 0.998 | 0.999 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 289 | 0.1 M Bis-Tris pH 5.5, 0.1 M ammonium acetate, 17% (w/v) PEG 10,000 |
