5LLJ

Maedi-Visna virus (MVV) integrase C-terminal domain (residues 220-276)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-02-28
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.97795
Spacegroup name	P 42 21 2
Unit cell lengths	64.250, 64.250, 72.330
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.470 - 1.780
R-factor	0.1883
Rwork	0.186
R-free	0.23360
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ex4
RMSD bond length	0.003
RMSD bond angle	0.668
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155:000))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.040	1.830
High resolution limit [Å]	1.780	1.780
Rmerge	0.057	1.470
Number of reflections	15111
<I/σ(I)>	23.7	1.5
Completeness [%]	99.9	99.4
Redundancy	12.2	9.9
CC(1/2)	1.000	0.520

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	291	0.2M potassium sodium tartrate 0.1M BTP pH 7.5 20% PEG-3350