5LL1

Crystal structure of urate oxidase from zebrafish

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-2
Synchrotron site	ESRF
Beamline	ID23-2
Temperature [K]	100
Detector technology	CCD
Collection date	2008-07-05
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.8726
Spacegroup name	P 1 21 1
Unit cell lengths	85.341, 127.415, 132.604
Unit cell angles	90.00, 102.21, 90.00

Refinement procedure

Resolution	64.803 - 2.800
R-factor	0.1997
Rwork	0.197
R-free	0.25750
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1r56
RMSD bond length	0.010
RMSD bond angle	1.412
Data reduction software	iMOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	83.300	2.950
High resolution limit [Å]	2.800	2.800
Rmerge	0.168	0.427
Number of reflections	64855
<I/σ(I)>	5.2	2.2
Completeness [%]	94.9	89
Redundancy	3.5	3.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	8.5	293	Tris-HCl 0.1M, 8% PEG 8000