5LL0
Structure of Polyphosphate Kinase 2 from Francisella tularensis SCHU S4 with polyphosphate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2013-10-13 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.92001 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 59.220, 145.620, 70.430 |
| Unit cell angles | 90.00, 113.23, 90.00 |
Refinement procedure
| Resolution | 59.142 - 1.960 |
| R-factor | 0.1999 |
| Rwork | 0.198 |
| R-free | 0.23940 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3czq |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | BALBES |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 72.810 |
| High resolution limit [Å] | 1.960 |
| Rmerge | 0.060 |
| Number of reflections | 76026 |
| <I/σ(I)> | 11.7 |
| Completeness [%] | 97.1 |
| Redundancy | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293.15 | 5 % Glycerol/PEG 4000, 0.1 M MES/imidazole pH 6.5, 0.15 M Morpheus alcohols, 1 mM polyP, 5 mM MgCl2 |
