5LKM
RadA bound to dTDP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-07-19 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9792 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 99.400, 169.900, 187.420 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.210 - 3.500 |
| R-factor | 0.18497 |
| Rwork | 0.183 |
| R-free | 0.22398 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5lko |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.823 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | SHELXDE |
| Refinement software | REFMAC (5.8.0124) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.210 | |
| High resolution limit [Å] | 3.500 | |
| Rmerge | 0.184 | 1.071 |
| Rmeas | 0.185 | 1.079 |
| Number of reflections | 20413 | |
| <I/σ(I)> | 22.76 | |
| Completeness [%] | 100.0 | |
| Redundancy | 66.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 291 | 200 mM MgF 100 mM HEPES pH8 |
